Scientific Laboratories

Eliava Institute houses seven scientific laboratories, an electron microscopy department and phage quality control group. Among scientific laboratories is the laboratory of bacterial strains and phage collection, where the institute keeps its vast collection of bacterial strains and phages.
The main goal of the ongoing research is the development of ecologically safe biopreparations and appropriate methodologies for their future application in human and animal health, agriculture and environmental protection.

The Laboratory of Applied Microbiology investigates problems of medical, veterinary and agricultural microbiology. The Laboratory is focused on creation of bacteriophage preparations for prevention and treatment of  infectious diseases.   From animal infectious diseases, we are interested in pathogens causing intestinal and purulent diseases (mastitis, endometrioses, salmonellosis, etc.), as well as in plant pathogens and creation of phage preparations against these pathogens.   The Laboratory is also focused on food safety issues, such as microbiological investigations of food products and epidemiological  research of food-borne pathogens for prevention of outbreaks of infections.   The Laboratory is focused on investigations of nosocomial pathogens and the elaboration of different phage-based methods for decontamination and elimination of such pathogens, especially biofilm formatting ones.   The Laboratory conducts investigations of microbial enzymes and creation of different drug preparations based on these enzymes.

Laboratory Personnel
Tarasi Gabisonia, MD – Head of the Laboratory – t.gabisonia@pha.ge
Manana Loladze, PhD – Leading Scientist – m.loladze@pha.ge
Natela Chakhunashvili, PhD – Leading Scientist
Manana Nadiradze, PhD – Senior Scientist
Teimuraz Katamadze, PhD – Scientist – t.katamadze@pha.ge
Maia Alibegashvili, MSc – Scientist
Tamar Kalandarishvili, MSc – Scientist
Tatiana Eliava – Scientist
Natia Tamarashvili– Scientist
Tinatin Kusiani – Senior laboratory assistant
Melania Kipiani – laboratory assistant
 
Scientific Grant projects (last 5 years)
 

  • 2019-2020 – Eliava Foundation – Association for the Development of Bacteriophages in Georgia – Study of the spread of pathogenic E.coli in poultry and selection of specific bacteriophages against them (PI: T. Katamadze)
  • 2017-2019– Shota Rustaveli National Science Foundation of Geoergia, Project – Application of Salmonella bacteriophages in models of infectious disease in chicken (PI: T. Gabisonia).
  • 2013-2015– GG-19-Ecology, genetic clustering and virulence of major bacterial and viral pathogens in the Republic of Georgia – Tularemia (PI: M. Kutateladze, Group Leader: T. Gabisonia).

 
Selected Publications (Last 5 years)
 

  • Gabisonia, T.; Giorgadze, I.; Topuria, N.; Chanishvili, L.; Nadiradze, M.; Chakhunashvili, N.; Katamadze, T.; Loladze, M.; Tamarashvili, N.; Alibegashvili, M.; et al. Fersis-Phage against Purulent-Inflammatory (Staphylococcal, Streptococcal) Pathologies. Arch clin Microbiol 2019, 10.
  • Gabisonia, T.; Giorgadze, I.; Topuria-Nakaidze, N.; Loladze, M.; Chakhunashvili, N.; Nadiradze, M.; Eliava, T.; Kalandarishvili, T.; Tamarashvili, N.; Katamadze, T. Characterization of New Bacteriophages of Salmonella and Shigella for Polivalent Intestinal “Enco Bacteriophage” Preparation. Microbiol Infect Dis 2019, 3, 1–3.
  • Gabisonia, T.; Loladze, M.; Chakhunashvili, N.; Tamarashvili, N.; Nadiradze, M.; Katamadze, T.; Eliava, T.; Kalandarishvili, T.; Alibegashvili, M. Bacteriophage Preparation for Treatment and Prevention of Salmonella Infection in Poultry. Journal of Agrarian Sciences 2019, 17, 36–44.
  • Gabisoniya, T.G.; Loladze, M.Z.; Nadiradze, M.M.; Chakhunashvili, N.K.; Alibegashvili, M.G.; Tamarashvili, N.G.; Pushkina, V.A. Effects of bacteriophages on biofilm formation by strains of Pseudomonas aeruginosa. Prikl Biokhim Mikrobiol 2016, 52, 312–317.

The Laboratory of Microbial Ecology studies different aspects of microbial ecology and evolution, including microbial diversity in natural and artificial environments, microenvironments of the human body, prevalent microflora in hospital environment, physiology of bacterial communities in relation to system-level processes, marine, freshwater and geothermal water microbial habitats, influence of environmental factors on abundance and composition of microbes and their viruses.   The Laboratory is actively engaged in the scientific projects related to the role of microbes in human health and disease, environmental and food safety, plant protection and aquaculture.   The phage research remains one of the main directions of the lab and is primarily associated with various aspects of phage ecology, also selection of efficient phage candidates for the prevention, diagnosis and therapy of bacterial diseases in medicine, food industry and agriculture.

Laboratory Personnel

Marine Tediashvili, PhD– Head of the Laboratory-m.tediashvili@pha.ge
Nino Janelidze, PhD – Leading Scientist -n.janelidze@pha.ge
Tamar Kokashvili, PhD – Senior Scientist – t.kokashvili@pha.ge
Tamar Eliashvili, PhD – Senior Scientist
Elene Didebulidze, PhD – Scientist – e.didebulidze @pha.ge  
Nino Lashkhi, MSc – Scientist
Ketevan Porchkhidze, MSc – Senior laboratory assistant
Tinatin Khukhunashvili, MSc – Senior laboratory assistant
Tamar Koberidze, MSc – Senior laboratry assistant
Kristina Tchalidze – Laboratory assistant
 
Scientific Grant projects (last 5 years)
 

  • 2019-2023– Ferring Pharmaceuticals: “Developments of Phage-based Products in the field of Reproductive Medicine and Women’s health”
  • STCU P664a 2018-2020 -Bacteriophages Lytic to selected multidrug resistant human pathogenic Bacteria: detailed characterization and evaluation of safety, therapeutic efficacy and decontamination potential
  • 2018-2020– CPEA-LT-2017/10061, “Network for research-based higher education in microbial biotechnology” (PI: N. Birkland Co. PI: E. Jaiani). 2017-2020, SRNSF FR 17-442, “Viruses in the Black Sea: diversity, community dynamics, virus-host interactions” (PI: E. Jaiani).
  • 2017-2019– CPEA-LT-2016/10095, “Network for improving research based higher education in basic and applied microbiology” (PI: N. Birkland Co. PI: E. Jaiani). 2016-2018, SRNSF PhD Fellowship, “Antibiotic resistance mechanisms in environmental Bacillus spp” (PI: E. Gabashvili).
  • 2016-2018– SRNSF-04/11-STCU -6209, “Phage-based environmentally safe strategy for control of bacterial infections in cultured freshwater fish species” (PI: N. Janelidze).
  • 2015-2018– STCU partner project P664, “Isolation and Detailed Characterization of Bacteriophages Lytic to Human Pathogenic Species Intrinsically Resistant to Antibiotics” (PI: M. Tediashvili).
  • 2015-2017– Shota Rustaveli National Science Foundation project № 460/10, “Study of the quarantine disease – Brown Rot of potato and elaborations of effective counter measures” (PI: G. Mepharishvili Co.PI: M. Tediashvili).
  • 2014-2016– UNICRI: Strengthening bio-safety and bio-security capabilities in South Caucasus and Central Asian countries project A4 – G. Eliava Institute, “Repairs and provision of equipment to essential laboratories of the Institute of Bacteriophages, Microbiology and Virology (IBMV) Georgia”, Work package P1++ “Purchasing of equipment and performance of upgrades essential for the Laboratory of Microbial Ecology (PI: M. kutateladze Co.PI: M. Tediashvili).
  • 2015-2017– CRDF-DTRA 60920, “Phage Mediated Antibiotic Resistance Gene Transfer in Marine, Freshwater and Extreme Environment” (PI: E. Jaiani).
  • 2014-2015– GRDF GLOBAL & Shota Rustaveli National Science Foundation (ECSP-2013), “Detection and characterization of hybrid biotypes among Georgian population of environmental isolates of V.cholerae” (PI: T. Kokashvili).
  • 2012-2014– STCU P450, “Screening for antiviral activity of indol-containing heterocyclic condensed systems for application in veterinary medicine” (PI: M. Tediashvili).
  • 2012-2013– GRDF GLOBAL – Mini Grant Program GMG 06/13, “Selection of Vibrio cholera specific bacteriophages for effective tracking down and control of new outbreaks of emerging infectious disease cholera” (PI: N. Janeldize).
  • 2011-2013– STCU P486, “Application of bacteriophages against highly pathogenic bacteria in model studies” (PI: M. Tediashvili).
  • 2011-2014– EU FP7, “Marie Courie PIRSES Network for development of phage therapy in aquaculture” (PI: P. Katharios Co.PI: M. Tediashvili).
  • 2010-2013– STCU-5148, “Development of a novel bacterial preparation for immunotherapy of cancer” (PI: K. Gambashidze Co.PI: M. Tediashvili).
  • 2009-2010– GRDF-GNSF/08-529, “Study of the diversity of Georgian environmental V.cholerae isolates by Eric-PCR and Phage typing techniques” (PI: T. Kokashvili).
  • 2008-2010– ISTC project 416.C, P.2, “Improvement of technology for preparation of anti-Anthrax immunoglobulin” (Phase II) (PI: D. Gogiashvili).

 
Selected Publications (Last 5 years)
 

  • Lasareishvili, B.; Shi, H.; Wang, X.; Hillstead, K.D.; Tediashvili, M.; Jaiani, E.; Tarabara, V.V. Virus Recovery by Tangential Flow Filtration: A Model to Guide the Design of a Sample Concentration Process. Biotechnol Prog 2021, 37, e3080, doi:10.1002/btpr.3080.
  • Jaiani, E.; Kusradze, I.; Kokashvili, T.; Geliashvili, N.; Janelidze, N.; Kotorashvili, A.; Kotaria, N.; Guchmanidze, A.; Tediashvili, M.; Prangishvili, D. Microbial Diversity and Phage-Host Interactions in the Georgian Coastal Area of the Black Sea Revealed by Whole Genome Metagenomic Sequencing. Mar Drugs 2020, 18, E558, doi:10.3390/md18110558.
  • Tserdze, T.; Jgenti, D.; Mgeladze, M.; Gambashidze, I.; Chkheidze, N.; Jaiani, E.; Didebulidze, E.; Janelidze, N.; Goradze, R.; Tediashvili, M. Dynamic Changes in the Upper Respiratory Tract Microflora of the Black Sea and Pacific Bottlenose Dolphins in Noogenic Habitat: Possible Influence of Environmental Conditions. IJAR 2018, 6, 1255–1263, doi:10.21474/IJAR01/6793.
  • Lashkhi, N.; Kokashvili, T.; Eliashvili, T.; Koberidze, T.; Tsertsvadze, G.; Muradashvili, M.; Meparishvili, G.; Sikharulidze, Z.; Tediashvili, M. The Isolation and Biological Characterization of Bacteriophages Lytic to Georgian Strains of Ralstonia solanacearum Race 3 Biovar 2. IJAR 2018, 6, 850–860, doi:10.21474/IJAR01/6503.
  • Tserodze, T.; Jgenti, D.; Mgeladze, M.; Chkheidze, N.; Lomidze, R.; Gambashidze, I.; Janelidze, N.; Didebulidze, E.; Groene, A.; Kik, M.; et al. Fatal Bacterial Infection of the Heart Incaptive Bottlenose Dolphin (Tursiops Truncatus Gilli), a Case Study. IJAR 2018, 6, 795–804, doi:10.21474/IJAR01/6285.
  • Koiava, T.; Gonçalves, D.; Palmeira, J.; Arobelidze, K.; Tediashvili, M.; Akhvlediani, L.; Ferreira, H. NDM Arrived to Republic of Georgia. J Glob Antimicrob Resist 2017, 8, 157–158, doi:10.1016/j.jgar.2017.01.001.
  • Koiava, T.; Gonçalves, D.; Palmeira, J.; Arobelidze, K.; Tediashvili, M.; Akhvlediani, L.; Ferreira, H. Phenotypic and Molecular Characterization of SHV, TEM, OXA and Extended Spectrum β-Lactamase Produced by Klebsiella pneumoniae Isolates in Adjara Hospital. International Journal of Current Research 2017, 8, 157–158.
  • Tserodze, T.; Zobova, N.; Jgenti, D.; Mgeladze, M.; Goradze, R.; Jaiani, E.; Didebulidze, E.; Tediashvili, M. Study of Water Hydrochemical and Microbiological Quality in the Noogenic Habitat of the Black See Bottlenose Dolphins. IJAR 2016, 4, 2063–2067, doi:10.21474/IJAR01/1690.
  • Expert round table on acceptance and re-implementation of bacteriophage therapy Silk Route to the Acceptance and Re-Implementation of Bacteriophage Therapy. Biotechnology Journal 2016, 11, 595–600, doi:10.1002/biot.201600023.

The Laboratory of Molecular Biology performs molecular and biological studies of the bacteriophages that are included in the Eliava Institute’s therapeutic and prophylactic preparations. The researchers of the laboratory have been involved in various scientific studies for the isolation and detailed characterization of bacteriophages against both aerobic and anaerobic bacteria. One of the main interests and activities of the team is investigation the mechanisms of phage – host bacterial cell interaction, study phage genes responsible for the lysis, and identification phage-specific “killer” proteins. The Laboratory has substantial experience and the capability for improvement and standardization of technological schemes for production of bacteriophage therapeutic preparations. Laboratory staff developed an exceptionally successful technology for production of highly purified Staphylococcal bacteriophage preparation.

Laboratory Personnel

Nana Balarjishvili, PhD – Head of the Laboratory – n.balarjishvili@pha.ge
Leila Kvachadze, PhD – Leading scientist – l.kvachadze@pha.ge
Elisabed Zaldastanishvili, Dr. rer. nat – Senior scientist – zaldastanishvili@pha.ge
Lika Leshkasheli – Scientist / Doctoral student – l.leshkasheli@pha.ge
Darejan Bolkvadze – Scientist / Doctoral student – d.bolkvadze@pha.ge
Lia Askilashvili – Senior Laboratory assistant – laskilashvili@pha.ge
Megi Khanishvili – Laboratory assistant- megi.xanishvili@gmail.com
Amalia Egikova – Laboratory assistant    
 
Scientific Grant projects (last 5 years)
 

  • 2019-2023 -Ferring Pharmaceuticals: Developments of Phage-based Products in the field of Reproductive Medicine and Women’s health (PI: M. Kutateladze)
  • 2018-2020– STCU P664a Bacteriophages Lytic to selected multidrug resistant human pathogenic Bacteria: detailed characterization and evaluation of safety, therapeutic efficacy and decontamination potential.
  • 2019-2020– Eliava Foundation- Association for the Development of Bacteriophages in Georgia- Evaluation of the effectiveness of phage therapy for the treatment of chronic infections (P.I. Nana Balarjishvili)
  • 2019-2020 -Eliava Foundation- Association for the Development of Bacteriophages in Georgia
    Study of MCR-1 gene induced colistin resistance in gram-negative bacteria isolated from Georgian and foreign patients (P.I. Darejan Bolkvadze).

  • 2018 -Eliava Foundation- Association for the Development of Bacteriophages in Georgia-
    Determination of the ability of Pseudomonas aeruginosa to produce biofilms by clinical strains and evaluating the action of bacteriophages and antibiotics on biofilm-associated bacteria.

  • 2018 – Eliava Foundation- Association for the Development of Bacteriophages in Georgia -Characterization of active, therapeutic, individual bacteriophages against Klebsiella pneumonia (P.I. Lika Leshkasheli).
  • 2014-2016– Scopes I Z7370_152319 – Evolution of a new Bacteriophage cocktail for treatment of Acinetobacter baumannii associated sepsis (PI: M. Kutateladze).

 
Selected Publications (Last 5 years)
 

  • Balarjishvili, N.; Kvachadze, L.; Tevdoradze, E.; Skhirtladze, N.; Leshkasheli, L.; Bolkvadze, D.; Pataridze, T.; Meskhi, T.; Chakraborty, R.; Kutateladze, M.; et al. Significance of Lysogeny for the Metabolism of Desulfovibrio Spp. Strains Isolated from Aquatic Environments of Georgia. J Appl Microbiol 2021, jam.15030, doi:10.1111/jam.15030.
  • Corbellino, M.; Kieffer, N.; Kutateladze, M.; Balarjishvili, N.; Leshkasheli, L.; Askilashvili, L.; Tsertsvadze, G.; Rimoldi, S.G.; Nizharadze, D.; Hoyle, N.; et al. Eradication of a Multidrug-Resistant, Carbapenemase-Producing Klebsiella pneumoniae Isolate Following Oral and Intra-Rectal Therapy With a Custom Made, Lytic Bacteriophage Preparation. Clinical Infectious Diseases 2020, 70, 1998–2001, doi:10.1093/cid/ciz782.
  • Leshkasheli, L.; Kutateladze, M.; Balarjishvili, N.; Bolkvadze, D.; Save, J.; Oechslin, F.; Que, Y.-A.; Resch, G. Efficacy of Newly Isolated and Highly Potent Bacteriophages in a Mouse Model of Extensively Drug-Resistant Acinetobacter baumannii Bacteraemia. Journal of Global Antimicrobial Resistance 2019, 19, 255–261, doi:10.1016/j.jgar.2019.05.005.
  • Sergueev, K.V.; Filippov, A.A.; Farlow, J.; Su, W.; Kvachadze, L.; Balarjishvili, N.; Kutateladze, M.; Nikolich, M.P. Correlation of Host Range Expansion of Therapeutic Bacteriophage Sb-1 with Allele State at a Hypervariable Repeat Locus. Appl Environ Microbiol 2019, 85, doi:10.1128/AEM.01209-19.
  • Farlow, J.; Bolkvadze, D.; Leshkasheli, L.; Kusradze, I.; Kotorashvili, A.; Kotaria, N.; Balarjishvili, N.; Kvachadze, L.; Nikolich, M.; Kutateladze, M. Genomic Characterization of Three Novel Basilisk-like Phages Infecting Bacillus anthracis. BMC Genomics 2018, 19, 685, doi:10.1186/s12864-018-5056-4.
  • Hoyle, N.; Zhvaniya, P.; Balarjishvili, N.; Bolkvadze, D.; Nadareishvili, L.; Nizharadze, D.; Wittmann, J.; Rohde, C.; Kutateladze, M. Phage Therapy against Achromobacter xylosoxidans Lung Infection in a Patient with Cystic Fibrosis: A Case Report. Research in Microbiology 2018, 169, 540–542, doi:10.1016/j.resmic.2018.05.001.
  • Antadze, I.; Dadunashvili, M.; Burbutashvili, T.; Gunia, S.; Balarjishvili, N.; Tevdoradze, E.; Pataridze, T.; Obiso, R.; Hagius, S.; Elzer, P.; et al. Diversity of Phage-Host Specificity in Brucella Phage. J Bacteriol Mycol 2017, 4, 1049.
  • Gundogdu, A.; Bolkvadze, D.; Kilic, H. In Vitro Effectiveness of Commercial Bacteriophage Cocktails on Diverse Extended-Spectrum Beta-Lactamase Producing Escherichia coli Strains. Front. Microbiol. 2016, 7, doi:10.3389/fmicb.2016.01761.
  • Kutateladze, M.; Leshkasheli, L.; Bolkvadze, D.; Askilashvili, L.; Balarjishvili, N. Bacteriophages against Multidrug Resistant Bacterial Infections. International Journal of Infectious Diseases 2016, 53, 46–47, doi:10.1016/j.ijid.2016.11.121.

Activity of the laboratory includes scientific research for human health and veterinary. The lab is working on study a humoral immunity status and protective immunoglobulins (IgA, IgG, IgM) for viral, bacterial and mixed infection. Correlation analysis of some representatives of the family of Herpes virus with other agents of non-viral ethology is one of the topics of the laboratory research.   Immune reactions of organisms in the process of therapeutic bacteriophage application, in particular, determination of non-specific and specific indicators of immunity are studied.   The groups is working on development of therapeutic-preventive immune-biological preparations (vaccines, sera).

Laboratory Personnel

Sergo Rigvava, PhD – Head of the Laboratory -s.rigvava@pha.ge
Nana topuria, PhD – Leading scientist – n.topuria@pha.ge
Dali Gogiashvili, PhD – Senior scientist  
Helen Akhalaia – Scientist – Akhalaia-nene@hotmail.com 
Nana Sabauri – Senior lab technician – nana_sabauri@hotmail.com  
Tamar Dalakishvili – lab technician – tamar.dalakishvili@yahoo.com
 
Scientific Grant projects (last 5 years)
 

  • 2019–2021 -Project Shota Rustaveli National Science Foundation of Geoergia AR-18-306 – Development of polyclonal immunoglobulins for treatment of complicated staphylococcal infections (PI: Sergo Rigvava).
  • 2013-2016– FP7 – Anthrax environmental decontamination network (PI: M. Kutateladze).

 
Selected Publications (Last 5 years)
 

  • Gubeladze, L.; Gogiashvili, D.; Rigvava, S.; Natidze, M.; Tsertsvadze, G.; Kamkamidze, M.; Tediashvili, M.; Kokashvili, T. The Isolation of Bacteriophages Lytic to B. anthracis from Environmental Sources in Georgia. 3 Rd Report: B. Anthracis –Specific Phages Isolated in 2012 in Georgia. WWJMRD 2018, 4, 1–4.
  • Rigvava, S.; Gubeladze, L.; Vardzelashvili, N.; Gogiashvili, D.; Natidze, M. Serological Express Diagnostics of Bacterial Sepsis. Georgian Critical Care Medicine 2018, 37.
  • Topuria-Nakaidze, N.; Giorgadze, I.; Odishelidze, N.; Sabauri, N.; Akhalaia, E.; Tkemaladze, L.; Katukia, T. Correlation Analyses between Herpes Simplex Virus and Other Pathogens Causing Infections in Females of Reproductive Age. Experimental and Clinical Medicine 2017, 4, 61–65.
  • Gubeladze, L.; Gogiashvili, D.; Rigvava, S.; Natidze, M.; Kamkamidze, M.; Tsertsvadze, G.; Tediashvili, M. Comparative Biological Characterization of Phages Specific to B.anthracis Isolated from Environmental Sources in Georgia. 2th Report. B. anthracis –Specific Phages Isolated in Georgia in 2010-2011. The Caucasus Multidisciplinary Journal 2017, 20, 53–58.
  • Abramia, G.; Golijashvili, A.; Rigvava, S. Bacteriophages against Antibiotic Resistant Salmonella Bacteria for the Possible Prevention and Treatment of Birds and Clean Up of Their Water Habitats. J Vet Sci Technol 2016, 07, doi:10.4172/2157-7579.1000395.

The main function of the Phage and Strains Collection is organization and centralized recording of all bacteriophages and bacterial strains held across the various laboratories of the G. Eliava Institute. Registration and storage of pathogenic and conditionally pathogenic bacterial strains and phages are based on study their biological properties that allows further expansion and development of collection for  scientific research and biotechnological production. The scientists of the laboratory participate in different scientific projects, which are focused on isolation and study of various phages against different aerobic and anaerobic microorganisms.

Laboratory Personnel  

Marine Darsavelidze, PhD – Head of the Laboratory – m.darsavelidze@pha.ge  
Tamar Suladze, PhD – Senior Scientist – tamuna_suladze@pha.ge  
Sophio Gunia, PhD – Scientist – guniasopo@hotmail.com
Maia Elizbarashvili, PhD – Scientist – m.elizbarashvili@pha.ge  
Marina Dadunashvili – Senior Laboratory assistant
Manana Labadze – Laboratory assistant  
Mzia Mchedlidze – Laboratory assistant
 
Scientific Grant projects (last 5 years)
 

  • 2019-2023– Ferring Pharmaceuticals – Developments of Phage-based Products in the field of Reproductive Medicine and Women’s health (PI: M.Kutateladze)
  • 2015-2018– Project 664 – Bacteriophages lytic to selected multidrug resistant human pathogenic bacteria: detailed characterization and evaluation of safety, therapeutic efficacy and decontamination potential (M. Darsavelidze, T.suladze)
  • 2015-2018– STCU P664 – Isolation, selection and characteristic of bacteriophages against antibiotic resistant no-fermentative microorganisms (PI: M. Tediashvili).

 
Selected Publications (Last 5 years)
 

  • Elizbarashvili, M.; Darsavelidze, M.; Suladze, T.; Gunia, S.; Dadunashvili, M. Analysis of Spectrum and Determination of Antibiotic Sensitivity of Causative Agents of Upper Respiratory Tract Infections. WWJMRD 2019, 5, 78–80.
  • Gunia, S.; Darsavelidze, M.; Suladze, T.; Elizbarashvili, M.; Dadunashvili, M. Distribution of Antibiotic-Resistant Staphylococcus Strains among Purulent-Inflammatory Infections. WWJMRD 2019, 5, 120–123.
  • Elizbarashvili, M.; Darsavelidze, M.; Suladze, T.; Gunia, S.; Dadunashvili, M. Study of Microbiocenosis of Intestine in Children of Different Age Category. WWJMRD 2018, 4, 372–374.
  • Antadze, I.; Dadunashvili, M.; Burbutashvili, T.; Gunia, S.; Balarjishvili, N.; Tevdoradze, E.; Pataridze, T.; Obiso, R.; Hagius, S.; Elzer, P.; et al. Diversity of Phage-Host Specificity in Brucella Phage. J Bacteriol Mycol 2017, 4, 1049.

Research and Development department was established in 2010 on the base of the former Laboratory of Genetics of Microorganisms. The main directions of scientific work are: research and development of bacteriophages active to various aerobic and anaerobic bacteria; isolation of lactic acid bacteria from endemic food products and assessment of their biotechnological value. The laboratory possesses large collection of well characterized bacteriophages and their host bacteria. The collection includes also lactic bacterial cultures that can be potentially used for construction of dairy starters.

Laboratory Personnel

Nina Chanishvili, PhD – Head of the Laboratory – nina.chanishvili@pha.ge
Gulnara Natroshvili, PhD – Senior Scientist
Nata Bakuradze – Scientist- nata.bakuradze@pha.ge
Nino Grdzelishvili – Scientist-n.grdzelishvili@pha.ge
Tamar Tatrishvili – Scientist-t.tatrishvili@pha.ge
Elene Javakhishvili – Senior Laboratory assistant
 
Scientific Grant projects (last 5 years)
 

  • 2019-2021 – Shota Rustaveli National Science Foundation of Geoergia # 17-17189 The perspectives of phage therapy for treatment of Autism Spectrum Disorders (ASD) in Georgian population (Co-manager: Nina Chansivili)
  • 2019-2021 – Shota Rustaveli National Science Foundation of Geoergia, Doctoral Study Program, # PHDF-19-8446: “Molecular and biological characterization of Pseudomonas aeruginosa specific bacteriophages and study of immune response to phage therapy using in vitro cells culture model” (PI: Nino Grzelishvili)
  • 2019-2021 – Eliava Foundation- Association for the Development of Bacteriophages in Georgia- Commercialization of the collection of the endemic lactic acid bacteria (PI: Nina Chanishvili)
  • 2018-2021 -Horizon 2020 FETOPEN #767015- CURE Constructing a ‘Eubiosis Reinstatement Therapy’ for Asthma (Co-manager: Nina Chanishvili)
  • 2015-2018 – ISTC A 2140 -Targeted elimination of MRD Salmonella with bacteriophages (Co-manager: Nina Chanishvili)
  • 2015-2017 – Shota Rustaveli National Science Foundation of Georgia FR/235/250-7/14 Use Bacteroides fragilis phages for prophylaxis of colon cancer: Assessment of its potential (PI: Nino Chanishvili)
  • 2016-2017 – Shota Rustaveli National Science Foundation of Georgia # IG/15/1/16- Bacteroides spp. special identification and detection of the enterotoxin genes (PI: Nata Bakuradze).
  • 2015-2016 – Shota Rustaveli National Science Foundation of Georgia # DO/311/7-280/14 – Study of probiotic properties of lactic acid bacteria isolated in Georgia, their genetic identification and development of database (PI: Elene Kakabadze).
  • 2014-2017 – Swiss National Foundation SCOPES program – Bacteriophage therapy for treating of urinary tract infections: A randomized, placebo supported double blind trial (PI: Nina Chanishvili)
  • 2014-2016 – CRDF Global – BPG -A-60792-Development of the commercial prototype for dairy products using endemic lactic acid bacteria: pilot technology experiment (PI: Nina Chansihvili)

 
Selected Publications (Last 5 years)
 

  • Leitner, L.; Ujmajuridze, A.; Chanishvili, N.; Goderdzishvili, M.; Chkonia, I.; Rigvava, S.; Chkhotua, A.; Changashvili, G.; McCallin, S.; Schneider, M.P.; et al. Intravesical Bacteriophages for Treating Urinary Tract Infections in Patients Undergoing Transurethral Resection of the Prostate: A Randomised, Placebo-Controlled, Double-Blind Clinical Trial. The Lancet Infectious Diseases 2021, 21, 427–436, doi:10.1016/S1473-3099(20)30330-3.
  • Makalatia, K.; Kakabadze, E.; Bakuradze, N.; Grdzelishvili, N.; Stamp, B.; Herman, E.; Tapinos, A.; Coffey, A.; Lee, D.; Papadopoulos, N.G.; et al. Investigation of Salmonella Phage-Bacteria Infection Profiles: Network Structure Reveals a Gradient of Target-Range from Generalist to Specialist Phage Clones in Nested Subsets. Viruses 2021, 13, 1261, doi:10.3390/v13071261.
  • Chanishvili, N.; Alavidze, Z. Early Therapeutic and Prophylactic Uses of Bacteriophages. In Bacteriophages; Harper, D.R., Abedon, S.T., Burrowes, B.H., McConville, M.L., Eds.; Springer International Publishing: Cham, 2020; pp. 1–30 ISBN 978-3-319-40598-8.
  • Leitner*, L.; Ujmajuridze, A.; Chanishvili, N.; Goderdzishvili, M.; Mehnert, U.; Chkhotua, A.; Sybesma, W.; Kessler, T.M. MP53-16 Adapted Bacteriophages for Treating Urinary Tract Infections. Journal of Urology 2019, 201, doi:10.1097/01.JU.0000556530.61448.e3.
  • Ngassam-Tchamba, C.; Duprez, J.N.; Fergestad, M.; De Visscher, A.; L’Abee-Lund, T.; De Vliegher, S.; Wasteson, Y.; Touzain, F.; Blanchard, Y.; Lavigne, R.; et al. In Vitro and in Vivo Assessment of Phage Therapy against Staphylococcus aureus Causing Bovine Mastitis. Journal of Global Antimicrobial Resistance 2020, 22, 762–770, doi:10.1016/j.jgar.2020.06.020.
  • Adriaenssens, E.M.; Sullivan, M.B.; Knezevic, P.; van Zyl, L.J.; Sarkar, B.L.; Dutilh, B.E.; Alfenas-Zerbini, P.; Łobocka, M.; Tong, Y.; Brister, J.R.; et al. Taxonomy of Prokaryotic Viruses: 2018-2019update from the ICTV Bacterial and Archaeal Viruses Subcommittee. Arch Virol 2020, 165, 1253–1260, doi:10.1007/s00705-020-04577-8.
  • Chanishvili, N. Commercial Products for Human Phage Therapy. In Bacterial Viruses: Exploitation for Biocontrol and Therapeutics; Caister Academic Press, 2020 ISBN 978-1-913652-51-7.
  • Chanishvili, N.; Nadareishvili, L.; Zaldastanishvili, E.; Balarjishvili, N.; Kutateladze, M. Application of Bacteriophages in Human Therapy: Recent Advances at the George Eliava Institute. In Bacterial Viruses: Exploitation for Biocontrol and Therapeutics; Caister Academic Press, 2020 ISBN 978-1-913652-51-7.
  • Issa, R.; Chanishvili, N.; Caplin, J.; Kakabadze, E.; Bakuradze, N.; Makalatia, K.; Cooper, I. Antibiofilm Potential of Purified Environmental Bacteriophage Preparations against Early Stage Pseudomonas aeruginosa Biofilms. J Appl Microbiol 2019, 126, 1657–1667, doi:10.1111/jam.14241.
  • Kurtböke, İ.; Chanishvili, N.; Barr, J.J. Bacteriophages. Microbiol. Aust. 2019, 40, 3, doi:10.1071/MA19002.
  • Chanishvili, N.; Aminov, R. Bacteriophage Therapy: Coping with the Growing Antibiotic Resistance Problem. Microbiol. Aust. 2019, 40, 5, doi:10.1071/MA19011.
  • Kakabadze, E.; Zago, M.; Rossetti, L.; Bonvini, B.; Tidona, F.; Carminati, D.; Chanishvili, N.; Giraffa, G. Characterisation of Lactic Acid Bacteria Isolated from the Georgian, Yoghurt‐like Matsoni. Int J Dairy Technol 2019, 1471–0307.12589, doi:10.1111/1471-0307.12589.
  • Kakabadze, E.; Makalatia, K.; Grdzelishvili, N.; Bakuradze, N.; Goderdzishvili, M.; Kusradze, I.; Phoba, M.-F.; Lunguya, O.; Lood, C.; Lavigne, R.; et al. Selection of Potential Therapeutic Bacteriophages That Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonella enterica Serovar Typhi Strain from the Democratic Republic of the Congo. Viruses 2018, 10, 172, doi:10.3390/v10040172.
  • Ujmajuridze, A.; Chanishvili, N.; Goderdzishvili, M.; Leitner, L.; Mehnert, U.; Chkhotua, A.; Kessler, T.M.; Sybesma, W. Adapted Bacteriophages for Treating Urinary Tract Infections. Front Microbiol 2018, 9, 1832, doi:10.3389/fmicb.2018.01832.
  • Kakabadze, E.; Makalatia, K.; Merabishvili, M.; Grdzelishvili, N.; Bakuradze, N.; Mkrtchyan, M.; Goderdzishvili, M.; Pirnay, J.-P.; Chanishvili, N.; Sedrykyan, A. Phage Typing, Antibiotic Resistance and Genomic Rep-PCR Fingerprinting of Clinical Salmonella Isolates from the Caucasus Region. International Journal of Infectious Diseases 2018, 73, 148, doi:10.1016/j.ijid.2018.04.3750.
  • Bakuradze, N.; Makalatia, K.; Merabishvili, M.; Togoshvili, L.; Chanishvili, N. Selection of the Active Phages against B. fragilis for Further Study of Therapeutic Perpectives. Georgian Med News 2018, 111–116.
  • Makalatia, Kh.; Kakabadze, E.; Bakuradze, N.; Grdzelishvili, N.; Natroshvili, G.; Kusradze, I.; Goderdzishvili, M.; Sedrakyan, A.; Arakelova, K.; Mkrtchyan, M.; et al. Activity of Bacteriophages to Multiply Resistant Strains of Salmonella and Their Various Serotypes. Veterinary biotechnology 2018, 32, 500–508, doi:10.31073/vet_biotech32(1)-67.
  • Gelman, D.; Eisenkraft, A.; Chanishvili, N.; Nachman, D.; Coppenhagem Glazer, S.; Hazan, R. The History and Promising Future of Phage Therapy in the Military Service. J Trauma Acute Care Surg 2018, 85, S18–S26, doi:10.1097/TA.0000000000001809.
  • Janashia, I.; Choiset, Y.; Jozefiak, D.; Déniel, F.; Coton, E.; Moosavi-Movahedi, A.A.; Chanishvili, N.; Haertlé, T. Beneficial Protective Role of Endogenous Lactic Acid Bacteria Against Mycotic Contamination of Honeybee Beebread. Probiotics & Antimicro. Prot. 2018, 10, 638–646, doi:10.1007/s12602-017-9379-2.
  • Aminov, R.; Caplin, J.; Chanishvili, N.; Coffey, A.; Cooper, I.; De Vos, D.; Doškař, J.; Friman, V.-P.; Kurtböke, İ.; Pantucek, R.; et al. Application of Bacteriophages. Microbiol. Aust. 2017, 38, 63, doi:10.1071/MA17029.
  • Janashia, I.; Choiset, Y.; Rabesona, H.; Hwanhlem, N.; Bakuradze, N.; Chanishvili, N.; Haertlé, T. Protection of Honeybee Apis Mellifera by Its Endogenous and Exogenous Lactic Flora against Bacterial Infections. Annals of Agrarian Science 2016, 14, 177–181, doi:10.1016/j.aasci.2016.07.002.
  • Janashia, I.; Carminati, D.; Rossetti, L.; Zago, M.; Fornasari, M.E.; Haertlé, T.; Chanishvili, N.; Giraffa, G. Characterization of Fructophilic Lactic Microbiota of Apis Mellifera from the Caucasus Mountains. Ann Microbiol 2016, 66, 1387–1395, doi:10.1007/s13213-016-1226-2.
  • Expert round table on acceptance and re-implementation of bacteriophage therapy Silk Route to the Acceptance and Re-Implementation of Bacteriophage Therapy. Biotechnology Journal 2016, 11, 595–600, doi:10.1002/biot.201600023.
  • sybesma, W.; Zbinden, R.; Chanishvili, N.; Kutateladze, M.; Chkhotua, A.; Ujmajuridze, A.; Mehnert, U.; Kessler, T.M. Bacteriophages as Potential Treatment for Urinary Tract Infections. Front. Microbiol. 2016, 7, doi:10.3389/fmicb.2016.00465.
  • Uimajuridze, A.; Jvania, G.; Chanishvili, N.; Goderdzishvili, M.; Sybesma, W.; Managadze, L.; Chkhotua, A.; Kessler, T. 265 Phage Therapy for the Treatment for Urinary Tract Infection: Results of in-Vitro Screenings and in-Vivo Application Using Commercially Available Bacteriophage Cocktails. European Urology Supplements 2016, 15, e265, doi:10.1016/S1569-9056(16)60267-1.

The main research interest and scientific activity of the laboratory is the isolation of bacteriophages against various pathogenic bacterial strains and study phage-biology and genetics, as well as the elaboration of different medicinal forms of phage preparations and development of technology for phage production. The laboratory personnel participate in various international scientific projects in the field of medicine, veterinary and microbial ecology. In addition to scientific-research directions, the laboratory is creating new therapeutic forms of phage preparations, developing and standardizing the technology of their production. The laboratory actively cooperates with the commercial structures of the Eliava-Consortium (“Bacteriophage Biopreparations”, “Pharmacy”, “Phage therapy Center”) both in terms of expanding the antimicrobial spectrum (activity) of existing commercial phage preparations, as well as preparing individual phages for patients.

laboratory Personnel

Marina Goderdzishvili, PhD – Head of the Laboratory – mgoderdzishvili@pha.ge  
Irina Chkonia, PhD – Leading Scientist –ichkonia@gtu.ge  
Ia Kusradze, PhD – Senior Scientist –iakusradze@pha.ge  
Natia Karumidze, PhD – Senior Scientist – natiaqarumidze@pha.ge
Sophie Rigvava, PhD – Senior Scientist –srigvava@pha.ge  
Teona Dvalidze – Scientist – tatiadvalidze@pha.ge
 
Nino Qvatadze – Senior laboratory assistant
Darejan Jgenti – Senior laboratory assistant
 
Scientific Grant projects (last 5 years)
 

  • 2020-2021 -GNSF – CARYS-19-378 – New Phage Pastilles Against Antibiotic Resistant Bacterial Infection of Oral Cavity (PI: Ia Kusradze)
  • 2017-2020– SRNSF FR 17-442 – Viruses in the Black Sea: diversity, community dynamics, virus-host interactions (PI: E. Jaiani).
  • 2019–2021 -Project SRNSF AR-18-306- Development of polyclonal immunoglobulins for treatment of complicated staphylococcal infections (PI: Sergo Rigvava)
  • 2019-2023– Ferring Pharmaceuticals: Developments of Phage-based Products in the field of Reproductive Medicine and Women’s health (PI: M.Kutateladze)
  • 2018-2020– SRNSF Research Grants for young scientists YS-18-413- Isolation and study of antibacterial effectiveness of phages against Group B Streptococcus (PI: S.Rigvava)
  • 2017-2021– H2020-FETOPEN 767015 – Constructing a Eubiosis Reinstatement Therapy for Asthma (Co PI: N. Chanishvili).
  • 2018-2021– STCU P664 – Bacteriophages lytic to selected multidrug resistant Human Pathogenic bacteria: Detailed Characterization and evaluation of safety therapeutic efficacy and decontamination potential of Bacteriophages Lytic to Species Intrinsically Resistant to Antibiotics (PI: M. Tediashvili)
  • 2016-2017– SRNSF 217874 – The role of XhIA domain containing protein in the development of lysogenic conversion (PI: S. Rigvava).
  • 2015-2017– ISTC A-1420 -Targeted elimination of multidrug-resistant (MDR) Salmonella by bacteriophages (Co PI: N. Chanishvili).
  • 2015-2018– STCU P664 – Isolation and Detailed Characterization of Bacteriophages Lytic to Human Pathogenic Species Intrinsically Resistant to Antibiotics (PI: M. Tediashvili).
  • 2015-2016 – Swiss Government Excellence Scholarship, University of Bern, Switzerland (PI: N. Karumidze)
  • 2014-2016– SNSF-SCOPES N 152304 – Bacteriophage therapy for treating urinary tract infections: A randomized placebo – controlled, double-blind trial (PI: N. Chanishvili).

 
Selected Publications (Last 5 years)
 

  • Kusradze, I.; Chkonia, I.; Karumidze, N.; Rigvava, S.; Dvalidze, T.; Barbaqadze, S.; Tatrishvili, N.; Bakuridze, A.; Bakuridze, L.; Goderdzishvili, M. New Phage Pastilles Against Antibiotic Resistant Bacterial Infection of Oral Cavity. Jiomics 2021, 11.
  • Rigvava, S.; Karumidze, N.; Kusradze, I.; Dvalidze, T.; Tatrishvili, N.; Goderdzishvili, M. Biological Characterization of Bacteriophages against Streptococcus agalactiae. Georgian Med News 2021, 182–186.
  • Leitner, L.; Ujmajuridze, A.; Chanishvili, N.; Goderdzishvili, M.; Chkonia, I.; Rigvava, S.; Chkhotua, A.; Changashvili, G.; McCallin, S.; Schneider, M.P.; et al. Intravesical Bacteriophages for Treating Urinary Tract Infections in Patients Undergoing Transurethral Resection of the Prostate: A Randomised, Placebo-Controlled, Double-Blind Clinical Trial. The Lancet Infectious Diseases 2021, 21, 427–436, doi:10.1016/S1473-3099(20)30330-3.
  • Jaiani, E.; Kusradze, I.; Kokashvili, T.; Geliashvili, N.; Janelidze, N.; Kotorashvili, A.; Kotaria, N.; Guchmanidze, A.; Tediashvili, M.; Prangishvili, D. Microbial Diversity and Phage-Host Interactions in the Georgian Coastal Area of the Black Sea Revealed by Whole Genome Metagenomic Sequencing. Mar Drugs 2020, 18, E558, doi:10.3390/md18110558.
  • Chanishvili, N. Commercial Products for Human Phage Therapy. In Bacterial Viruses: Exploitation for Biocontrol and Therapeutics; Caister Academic Press, 2020 ISBN 978-1-913652-51-7.
  • Ujmajuridze, A.; Chanishvili, N.; Goderdzishvili, M.; Leitner, L.; Mehnert, U.; Chkhotua, A.; Kessler, T.M.; Sybesma, W. Adapted Bacteriophages for Treating Urinary Tract Infections. Front Microbiol 2018, 9, 1832, doi:10.3389/fmicb.2018.01832.
  • Rigvava, S.; Kharebava, S.; Giorgobiani, T.; Dvalidze, T.; Goderdzishvili, M. Identification and Antibiotic Susceptibility Patterns of Streptococcus agalactiae. Georgian Med News 2019, 149–153.
  • Kakabadze, E.; Makalatia, K.; Merabishvili, M.; Grdzelishvili, N.; Bakuradze, N.; Mkrtchyan, M.; Goderdzishvili, M.; Pirnay, J.-P.; Chanishvili, N.; Sedrykyan, A. Phage Typing, Antibiotic Resistance and Genomic Rep-PCR Fingerprinting of Clinical Salmonella Isolates from the Caucasus Region. International Journal of Infectious Diseases 2018, 73, 148, doi:10.1016/j.ijid.2018.04.3750.
  • Makalatia, Kh.; Kakabadze, E.; Bakuradze, N.; Grdzelishvili, N.; Natroshvili, G.; Kusradze, I.; Goderdzishvili, M.; Sedrakyan, A.; Arakelova, K.; Mkrtchyan, M.; et al. Activity of Bacteriophages to Multiply Resistant Strains of Salmonella and Their Various Serotypes. Veterinary biotechnology 2018, 32, 500–508, doi:10.31073/vet_biotech32(1)-67.
  • Kakabadze, E.; Makalatia, K.; Grdzelishvili, N.; Bakuradze, N.; Goderdzishvili, M.; Kusradze, I.; Phoba, M.-F.; Lunguya, O.; Lood, C.; Lavigne, R.; et al. Selection of Potential Therapeutic Bacteriophages That Lyse a CTX-M-15 Extended Spectrum β-Lactamase Producing Salmonella enterica Serovar Typhi Strain from the Democratic Republic of the Congo. Viruses 2018, 10, 172, doi:10.3390/v10040172.
  • Rigvava, S.; Kusradze, I.; Karumidze, N.; Dvalidze, T.; Katsitadze, M.; Barbakadze, S.; Bolkvadze, D.; Goderdzishvili, M. New Temperate Enterococcus Phage VB_GEC_EFS_2 with Potential of Lysogenic Conversion. Georgian Med News 2018, 158–164.
  • Leitner, L.; Sybesma, W.; Chanishvili, N.; Goderdzishvili, M.; Chkhotua, A.; Ujmajuridze, A.; Schneider, M.P.; Sartori, A.; Mehnert, U.; Bachmann, L.M.; et al. Bacteriophages for Treating Urinary Tract Infections in Patients Undergoing Transurethral Resection of the Prostate: A Randomized, Placebo-Controlled, Double-Blind Clinical Trial. BMC Urol 2017, 17, 90, doi:10.1186/s12894-017-0283-6.
  • Nogueira, F.; Karumidze, N.; Kusradze, I.; Goderdzishvili, M.; Teixeira, P.; Gouveia, I.C. Immobilization of Bacteriophage in Wound-Dressing Nanostructure. Nanomedicine 2017, 13, 2475–2484, doi:10.1016/j.nano.2017.08.008.
  • Kusradze, I.; Karumidze, N.; Rigvava, S.; Dvalidze, T.; Katsitadze, M.; Amiranashvili, I.; Goderdzishvili, M. Characterization and Testing the Efficiency of Acinetobacter baumannii Phage VB-GEC_Ab-M-G7 as an Antibacterial Agent. Front Microbiol 2016, 7, 1590, doi:10.3389/fmicb.2016.01590.
  • Uimajuridze, A.; Jvania, G.; Chanishvili, N.; Goderdzishvili, M.; Sybesma, W.; Managadze, L.; Chkhotua, A.; Kessler, T. 265 Phage Therapy for the Treatment for Urinary Tract Infection: Results of in-Vitro Screenings and in-Vivo Application Using Commercially Available Bacteriophage Cocktails. European Urology Supplements 2016, 15, e265, doi:10.1016/S1569-9056(16)60267-1.